Publication
Title
In vitro cell wall extensibility controls age-related changes in the growth rate of etiolated **Arabidopsis** hypocotyls
Author
Abstract
Plant cell growth is controlled by cell wall extensibility, which is currently estimated indirectly by various microtensile and nano/microindentation techniques. Their outputs differ in the accuracy of growth rate and in vivo extensibility prediction. Using the creep method we critically tested several metrics (creep rate, creep rate × stress1, in vitro cell wall extensibility (ϕ) and in vitro cell wall yield threshold (y)) for their ability to predict growth rates of etiolated Arabidopsis thaliana (L. Heynh.) hypocotyls. We developed novel approaches for ϕ and y determination and statistical analysis based on creep measurements under single loads coupled with wall stress calculation. The best indicator of growth rate was ϕ because the 3-fold developmental decrease in the growth rate of 4- vs 3-day-old hypocotyls was accompanied by a 3-fold decrease in ϕ determined at pH 5. Although the acid-induced expansin-mediated creep of cell walls resulted exclusively from increasing ϕ values, the decrease in ϕ between 3- and 4-day-old hypocotyls was not mediated by a decrease in expansin abundance. We give practical recommendations on the most efficient use of creep rate, creep rate × stress1, ϕ and y in different experimental situations and provide scripts for their automated calculations and statistical comparisons.
Language
English
Source (journal)
Functional plant biology. - Chippendale, N.S.W.
Publication
Chippendale, N.S.W. : CSIRO (Commonwealth Scientific and Industrial Research Organization), 2015
ISSN
1445-4408
Volume/pages
42:11(2015), p. 1068-1079
ISI
000369522800006
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
[E?say:metaLocaldata.cgzprojectinf]
Exploiting mutants to study the control of cell elongation in Arabidopsis thaliana hypocotyls.
Regulation of cell elongation during Arabidopsis thaliana development.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 19.12.2015
Last edited 21.08.2017
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