Title
Gold nanodome-patterned microchips for intracellular surface-enhanced Raman spectroscopy Gold nanodome-patterned microchips for intracellular surface-enhanced Raman spectroscopy
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Cambridge ,
Subject
Chemistry
Source (journal)
The analyst. - Cambridge
Volume/pages
140(2015) :24 , p. 8080-8087
ISSN
0003-2654
ISI
000365233300002
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
While top-down substrates for surface-enhanced Raman spectroscopy (SERS) offer outstanding control and reproducibility of the gold nanopatterns and their related localized surface plasmon resonance, intracellular SERS experiments heavily rely on gold nanoparticles. These nanoparticles often result in varying and uncontrollable enhancement factors. Here we demonstrate the use of top-down gold-nanostructured microchips for intracellular sensing. We develop a tunable and reproducible fabrication scheme for these microchips. Furthermore we observe the intracellular uptake of these structures, and find no immediate influence on cell viability. Finally, we perform a proof-of-concept intracellular SERS experiment by the label-free detection of extraneous molecules. By bringing top-down SERS substrates to the intracellular world, we set an important step towards time-dependent and quantitative intracellular SERS.
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Full text (open access)
https://repository.uantwerpen.be/docman/irua/72fd36/130334.pdf
https://repository.uantwerpen.be/docman/irua/080ec0/5a893ae5.pdf
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