Publication
Title
Comparison of conventional and capillary electrospray LC-MS and LC MS/MS for the analysis of DNA-adducts
Author
Abstract
Capillary liquid chromatography (LC) was coupled to electrospray mass spectrometry (MS) in order to improve the absolute detection limits required for the analysis of DNA adducts. The capillary LC/MS system was compared with conventional LC/MS system using 2'-deoxyadenosine (dAdo). The absolute detection limit could be improved by a factor 177, leading to the detection of 600 fg of dAdo under single-ion monitoring (SIM) conditions. Using capillary LC/MS, 1 pg of a bisphenol A diglycidyl ether-dAdo addut could be detected under SIM conditions. Full-scan low-energy collision-activated decomposition (CAD) spectra of 550 pg adduct are presented. Capillary LC/MS and capillary LC/MS/MS data are presented for in vitro reaction mixtures of 2'-deoxynucleosides and bisphenol A diglycidyl ether, a potent carcinogen.
Language
English
Source (journal)
Journal of mass spectrometry. - Chichester
Publication
Chichester : 1995
ISSN
1076-5174
Volume/pages
(1995), p. 143-152
ISI
A1995UG39900020
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 08.10.2008
Last edited 21.04.2017
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