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Title
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Comparison of conventional and capillary electrospray LC-MS and LC MS/MS for the analysis of DNA-adducts
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Author
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Abstract
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| Capillary liquid chromatography (LC) was coupled to electrospray mass spectrometry (MS) in order to improve the absolute detection limits required for the analysis of DNA adducts. The capillary LC/MS system was compared with conventional LC/MS system using 2'-deoxyadenosine (dAdo). The absolute detection limit could be improved by a factor 177, leading to the detection of 600 fg of dAdo under single-ion monitoring (SIM) conditions. Using capillary LC/MS, 1 pg of a bisphenol A diglycidyl ether-dAdo addut could be detected under SIM conditions. Full-scan low-energy collision-activated decomposition (CAD) spectra of 550 pg adduct are presented. Capillary LC/MS and capillary LC/MS/MS data are presented for in vitro reaction mixtures of 2'-deoxynucleosides and bisphenol A diglycidyl ether, a potent carcinogen. |
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Language
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English
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Source (journal)
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Journal of mass spectrometry. - Chichester
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Publication
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Chichester
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1995
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ISSN
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1076-5174
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Volume/pages
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(1995)
, p. 143-152
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ISI
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A1995UG39900020
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