Title
Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and functionDeiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Research group
Systemic Physiological and Ecotoxicological Research (SPHERE)
Veterinary physiology and biochemistry
Publication type
article
Publication
Amsterdam,
Subject
Biology
Veterinary medicine
Human medicine
Source (journal)
Molecular and cellular endocrinology. - Amsterdam
Volume/pages
424(2016):C, p. 81-93
ISSN
0303-7207
ISI
000372675200008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Retinal development in vertebrates relies extensively on thyroid hormones. Their local availability is tightly controlled by several regulators, including deiodinases (Ds). Here we used morpholino technology to explore the roles of Ds during eye development in zebrafish. Transcriptome analysis at 3 days post fertilization (dpf) revealed a pronounced effect of knockdown of both T4-activating Ds (D1D2MO) or knockdown of T3-inactivating D3 (D3bMO) on phototransduction and retinoid recycling. This was accompanied by morphological defects (studied from 1 to 7 dpf) including reduced eye size, disturbed retinal lamination and strong reduction in rods and all four cone types. Defects were more prominent and persistent in D3-deficient fish. Finally, D3-deficient zebrafish larvae had disrupted visual function at 4 dpf and were less sensitive to a light stimulus at 5 dpf. These data demonstrate the importance of TH-activating and -inactivating Ds for correct zebrafish eye development, and point to D3b as a central player.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/b8e5a6/131455.pdf
E-info
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