Title
An effective plasma membrane proteomics approach for small tissue samples
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Sciences. Chemistry
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
London :Nature Publishing Group ,
Subject
Biology
Human medicine
Source (journal)
Scientific reports. - London, 2011, currens
Volume/pages
5(2015) , p. 1-18
ISSN
2045-2322
Article Reference
10917
Carrier
E-only publicatie
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm3-scale tissue.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/aa669c/131773.pdf
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