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Title
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Crosslinking strategies for porous gelatin scaffolds
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Author
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Abstract
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| The present work reports on the application and the evaluation of a multitude of crosslinking approaches including high-energy irradiation, redox-initiating systems and conventional carbodiimide-coupling chemistry for frozen and/or freeze-dried porous gelatin scaffolds. The latter is particularly relevant for a plethora of biomedical applications such as tissue engineering supports, wound dressings, adhesive and absorbent pads for surgery, etc. Moreover, the results obtained for gelatin can be considered a proof-of-concept to be extrapolated to other polymer systems containing double bonds and/or amines and carboxylic acids to also realize scaffold crosslinking in dry or frozen state. The results showed that high-energy irradiation at -5 A degrees C enabled sufficient segmental mobility to induce chemical crosslinking after performing a cryogenic treatment of methacrylamide-modified gelatin scaffolds. Alternatively, although several redox-initiating systems were unable to chemically crosslink functionalized gelatin, the combination of ammonium persulphate and TEMED resulted in the formation of scaffolds with a reasonable gel fraction. Interestingly, carbodiimide-coupling was found suitable to crosslink freeze-dried gelatin matrices. |
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Language
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English
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Source (journal)
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Journal of materials science. - London
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Publication
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London
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2016
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ISSN
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0022-2461
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DOI
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10.1007/S10853-016-9747-4
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Volume/pages
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51
:9
(2016)
, p. 4349-4357
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ISI
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000370342100016
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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