Fast and reliable quantitative peptidomics with labelpepmatchFast and reliable quantitative peptidomics with labelpepmatch
Faculty of Sciences. Biology
Systemic Physiological and Ecotoxicological Research (SPHERE)
Journal of proteome research
15(2016):3, p. 1080-1089
University of Antwerp
The use of stable isotope tags in quantitative peptidomics offers many advantages, but the laborious identification of matching sets of labeled peptide peaks is still a major bottleneck. Here we present labelpepmatch, an R package for fast and straightforward analysis of LC MS spectra of labeled peptides. This open-source tool offers fast and accurate identification of peak pairs alongside an appropriate framework for statistical inference on quantitative peptidomics data, based on techniques from other-omics disciplines. A relevant case study on the desert locust Schistocerca gregaria proves our pipeline to be a reliable tool for quick but thorough explorative analyses.