Title
Survey of metallo-<tex>$\beta$</tex>-lactamase-producing Enterobacteriaceae colonizing patients in European ICUs and rehabilitation units, 2008-11 Survey of metallo-<tex>$\beta$</tex>-lactamase-producing Enterobacteriaceae colonizing patients in European ICUs and rehabilitation units, 2008-11
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London ,
Subject
Biology
Pharmacology. Therapy
Human medicine
Source (journal)
The journal of antimicrobial chemotherapy. - London, 1975, currens
The journal of antimicrobial chemotherapy. - London, 1975, currens
Volume/pages
70(2015) :7 , p. 1981-1988
ISSN
0305-7453
ISI
000359722900009
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Objectives. To perform a multinational survey of patients colonization by metallo-β-lactamase (MBL)-producing Enterobacteriaceae, including their molecular characterization. Methods. Patients in 18 hospital units across Europe and Israel (n=17,947) were screened between mid-2008 and mid-2011. MBL-producing isolates were typed by PFGE and MLST. MBL genes were amplified and sequenced within their integrons. Plasmids with MBL genes were analysed by nuclease S1 plus hybridisation profiling, mating and transformation assays, and by PCR-based replicon typing. Results. Ninety-one patients in nine centres (six countries) carried 94 non-duplicate MBL-producing organisms, including 62 patients in two Greek ICUs. Klebsiella pneumoniae isolates from Greece dominated (n=56) and belonged mainly to ST147, ST36 and ST383. All but one of the isolates expressed VIM-1 type MBLs. Isolates of Greek origins produced five enzymes, including new VIM-39, encoded by class 1 integrons of four types. In-e541-like elements prevailed, comprising six variants located on IncR, IncFIIK, IncR+FIIK, IncR+A/C or non-typeable plasmids. The other group were new In4873 and In4863, being first In416-like elements identified in Greece, present on IncA/C or non-typeable plasmids. Isolates from other countries produced only VIM-1 and the major integron was In916 identified in 16 organisms from France, Italy and Spain. In916 was carried by four plasmid types, including IncA/C, IncFIIK, and IncHI2. Other integrons included a new element In3103 in Spain, and In110 identified only in Latvia. Conclusions. The study provided fully comparable data on occurrence and molecular characteristics of VIM-producing Enterobacteriaceae in a group of hospital units across Europe, documenting recent changes in their epidemiology.
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Full text (open access)
https://repository.uantwerpen.be/docman/irua/15bd85/95320be2.pdf
E-info
https://repository.uantwerpen.be/docman/iruaauth/19f65e/7ec10605.pdf
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