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Title
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Attaching redox proteins onto electrode surfaces by bis-silane
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Author
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Abstract
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| Immobilization of redox proteins on electrode surfaces is of special interest for mechanistic studies and applications because of a well-controlled redox state of protein molecules by a polarized electrode and fast electron transfer kinetics, free from diffusion limitation. Here, bis-organosilane (1,2-bis(trimethoxysilyl)ethane) was applied as a fresh solution in a pH 7 phosphate buffer without use of any organic solvent, sol-gel or mesoporous bulk matrix. A short aging period of 30 minutes before deposition on the electrodes was optimal for the immobilization of proteins. Three redox proteins (cytochrome c, neuroglobin and GLB-12) were confined to the gold surface of electrodes with high coverages and stability, indicating that the suggested technique is simple, efficient and generic in nature. |
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Language
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English
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Source (journal)
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ChemElectroChem. - Place of publication unknown, 2013, currens
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Publication
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Place of publication unknown
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Wiley
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2016
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ISSN
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2196-0216
[online]
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DOI
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10.1002/CELC.201600021
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Volume/pages
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3
:7
(2016)
, p. 1035-1038
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ISI
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000380043500001
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Full text (Publisher's DOI)
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Full text (open access)
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