Publication
Title
Attaching redox proteins onto electrode surfaces by bis-silane
Author
Abstract
Immobilization of redox proteins on electrode surfaces is of special interest for mechanistic studies and applications because of a well-controlled redox state of protein molecules by a polarized electrode and fast electron transfer kinetics, free from diffusion limitation. Here, bis-organosilane (1,2-bis(trimethoxysilyl)ethane) was applied as a fresh solution in a pH 7 phosphate buffer without use of any organic solvent, sol-gel or mesoporous bulk matrix. A short aging period of 30 minutes before deposition on the electrodes was optimal for the immobilization of proteins. Three redox proteins (cytochrome c, neuroglobin and GLB-12) were confined to the gold surface of electrodes with high coverages and stability, indicating that the suggested technique is simple, efficient and generic in nature.
Language
English
Source (journal)
ChemElectroChem. - Place of publication unknown, 2013, currens
Publication
Place of publication unknown : Wiley, 2016
ISSN
2196-0216 [online]
Volume/pages
3:7(2016), p. 1035-1038
ISI
000380043500001
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 12.04.2016
Last edited 10.07.2017
To cite this reference