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Title
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LC-MS metabolomics from study design to data-analysis using a versatile pathogen as a test case
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Author
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Abstract
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| Thanks to significant improvements in LC-MS technology, metabolomics is increasingly used as a tool to discriminate the responses of organisms to various stimuli or drugs. In this minireview we discuss all aspects of the LC-MS metabolomics pipeline, using a complex and versatile model organism, Leishmania donovani, as an illustrative example. The benefits of a hyphenated mass spectrometry platform and a detailed overview of the entire experimental pipeline from sampling, sample storage and sample list set-up to LC-MS measurements and the generation of meaningful results with state-of-the-art data-analysis software will be thoroughly discussed. Finally, we also highlight important pitfalls in the processing of LC-MS data and comment on the benefits of implementing metabolomics in a systems biology approach. |
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Language
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English
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Source (journal)
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Computational and Structural Biotechnology Journal
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Publication
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2013
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ISSN
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2001-0370
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DOI
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10.5936/CSBJ.201301002
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Volume/pages
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4
:5
(2013)
, p. 1-8
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Article Reference
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e201301002
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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