Publication
Title
A large insertion in intron 2 of the TYRP1 gene associated with **American Palomino** phenotype in American mink
Author
Abstract
A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b P b P ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for amplifying the genomic DNA and cDNA at the beginning of intron 2 of AP TYRP1 revealed the presence of a large insertion of approximately eight kb. The insertion most likely disrupts different elements necessary for the splicing of intron 2 of the TYRP1 gene. In AP RNAseq data indicate, however, the presence of the wild-type (wt) transcript at very low levels and Western blot reveals three products when using an antibody raised against middle part of the TYRP1 protein. One individual from another brown mink phenotypecommercially named Dawnwas also investigated at the molecular level by long-range PCR and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.
Language
English
Source (journal)
Mammalian genome. - New York
Publication
New York : 2016
ISSN
0938-8990
Volume/pages
27:3-4(2016), p. 135-143
ISI
000373308200004
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 02.05.2016
Last edited 13.08.2017
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