Title
A large insertion in intron 2 of the TYRP1 gene associated with **American Palomino** phenotype in American mink A large insertion in intron 2 of the TYRP1 gene associated with **American Palomino** phenotype in American mink
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York ,
Subject
Chemistry
Biology
Veterinary medicine
Human medicine
Engineering sciences. Technology
Source (journal)
Mammalian genome. - New York
Volume/pages
27(2016) :3-4 , p. 135-143
ISSN
0938-8990
ISI
000373308200004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b P b P ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for amplifying the genomic DNA and cDNA at the beginning of intron 2 of AP TYRP1 revealed the presence of a large insertion of approximately eight kb. The insertion most likely disrupts different elements necessary for the splicing of intron 2 of the TYRP1 gene. In AP RNAseq data indicate, however, the presence of the wild-type (wt) transcript at very low levels and Western blot reveals three products when using an antibody raised against middle part of the TYRP1 protein. One individual from another brown mink phenotypecommercially named Dawnwas also investigated at the molecular level by long-range PCR and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/5275a3/133043.pdf
E-info
https://repository.uantwerpen.be/docman/iruaauth/45d55a/133043.pdf
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