Title
Pili of Lactobacillus rhamnosus GG mediate interaction with beta-lactoglobulin Pili of Lactobacillus rhamnosus GG mediate interaction with beta-lactoglobulin
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Oxford ,
Subject
Chemistry
Pharmacology. Therapy
Source (journal)
Food hydrocolloids. - Oxford
Volume/pages
58(2016) , p. 35-41
ISSN
0268-005X
ISI
000373168200005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Recently dairy products like cheese, yogurt or milk proteins are increasingly used to encapsulate probiotic bacteria to promote their survival. The knowledge of interactions occurring between bacteria and milk matrices is essential for the formulation of efficient new products. In this work, Atomic Force Microscopy was used to study interactions between Lactobacillus rhamnosus GG and isolated whey proteins: beta-lactoglobulin (beta-LG), alpha-lactalbumin (alpha-LA) and bovine serum albumin (BSA). For the first time, it was clearly demonstrated that L. rhamnosus GG is able to interact with the beta-LG but not with alpha-LA and BSA. To identify the bacterial surface biomolecules involved in interaction with beta-LG, two of its surface mutants were used: a pili depleted strain (L. rhamnosus GG spaCBA) and an exopolysaccharides (EPS) depleted strain (L. rhamnosus GG welE). Two predictive models, FJC and WLC, were used to describe behaviors of the molecule stretched by providing parameters like contour length (L-c), adhesion force (F-adh), Kuhn length (l(k)) and Persistence length (l(p)). The absence of adhesion events between beta-LG and mutants having no pili reflects the crucial role of this biomolecule for interactions with the protein. (C) 2016 Elsevier Ltd. All rights reserved.
E-info
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000373168200005&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000373168200005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
https://repository.uantwerpen.be/docman/iruaauth/4a2e92/133221.pdf
Handle