Title
Peripheral blood collection : the first step towards gene expression profiling Peripheral blood collection : the first step towards gene expression profiling
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Subject
Biology
Pharmacology. Therapy
Engineering sciences. Technology
Source (journal)
Biomarkers: biochemical indicators of exposure, response, and susceptibility to chemicals
Volume/pages
21(2016) :5 , p. 458-465
ISSN
1354-750X
ISI
000379348200010
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
A crucial challenge for gene expression analysis in human biomonitoring studies on whole blood samples is rapid sample handling and mRNA stabilization. This study was designed to evaluate the impact of short bench times (less than 30 min) on yield, quality and gene expression of mRNA in the presence of different stabilization buffers (TempusTM Blood RNA tube and RNAlater® Stabilization Reagent). Microarray analyzes showed significant changes over short periods of time in expression of a considerate part of the transcriptome (2356 genes) with a prominent role for NFкB-, cancer- and glucocorticoid-mediated networks, and specifically interleukin-8 (IL-8). These findings suggest that even short bench times affect gene expression, requiring to carry out blood collection in a strictly standardized way.
E-info
https://repository.uantwerpen.be/docman/iruaauth/d340e3/133455.pdf
Full text (open access)
https://repository.uantwerpen.be/docman/irua/ab8460/133455.pdf
E-info
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Handle