Title
Generation and cryopreservation of clinical grade Wilms' tumor 1 mRNA-loaded dendritic cell vaccines for cancer immunotherapy Generation and cryopreservation of clinical grade Wilms' tumor 1 mRNA-loaded dendritic cell vaccines for cancer immunotherapy
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Totowa, N.J. ,
Subject
Human medicine
Source (journal)
Methods in molecular biology. - Totowa, N.J., 1984, currens
Volume/pages
1393(2016) , p. 27-35
ISSN
1064-3745
1940-6029
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
First described in the seventies, dendritic cells (DC) are currently the subject of intense investigation to exploit their unique antigen-presenting and immunoregulatory capacities. In cancer, DC show promise to elicit or amplify immune responses directed against cancer cells by activating natural killer (NK) cells and tumor antigen-specific T cells. Wilms tumor 1 (WT1) protein is a tumor-associated antigen that is expressed in a majority of cancer types and has been designated as an antigen of major interest to be targeted in clinical cancer immunotherapy trials. In this chapter, we describe the generation, cryopreservation and thawing of clinical grade autologous monocyte-derived DC vaccines that are loaded with WT1 by messenger RNA (mRNA) electroporation. This in-house developed transfection method gives rise to presentation of multiple antigen epitopes and can be used for all patients without restriction of human leukocyte antigen (HLA) type.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/5bf1fc/133550.pdf
Handle