Title
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Characterizing the in vitro biofilm phenotype of **Staphylococcus epidermidis** isolates from central venous catheters
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Author
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Abstract
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Central venous catheter (CVC)-related infections are commonly caused by Staphylococcus epidermidis that is able to form a biofilm on the catheter surface. Many studies involving biofilm formation by Staphylococcus have been published each adopting an own in vitro model. Since the capacity to form a biofilm depends on multiple environmental factors, direct comparison of results obtained in different studies remains challenging. This study characterized the phenotype (strong versus weak biofilm-producers) of S. epidermidis from CVCs in four different in vitro biofilm models, covering differences in material type (glass versus polymer) and nutrient presentation (static versus continuous flow). A good correlation in phenotype was obtained between glass and polymeric surfaces independent of nutrient flow, with 85% correspondence under static growth conditions and 80% under dynamic conditions. A 80% correspondence between static and dynamic conditions on polymeric surfaces could be demonstrated as well. Incubation time had a significant influence on the biofilm phenotype with only 55% correspondence between the dynamic models at different incubation times (48 h versus 17 h). Screening for the presence of biofilm-related genes only revealed that ica A was correlated with biofilm formation under static but not under dynamic conditions. In conclusion, this study highlights that a high level of standardization is necessary to interpret and compare results of different in vitro biofilm models. |
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Language
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English
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Source (journal)
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Journal of microbiological methods. - Amsterdam
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Publication
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Amsterdam
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2016
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ISSN
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0167-7012
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DOI
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10.1016/J.MIMET.2016.05.009
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Volume/pages
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127
(2016)
, p. 95-101
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ISI
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000380624800017
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Pubmed ID
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27196636
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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