Title
Characterizing the in vitro biofilm phenotype of **Staphylococcus epidermidis** isolates from central venous catheters Characterizing the in vitro biofilm phenotype of **Staphylococcus epidermidis** isolates from central venous catheters
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Journal of microbiological methods. - Amsterdam
Volume/pages
127(2016) , p. 95-101
ISSN
0167-7012
ISI
000380624800017
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Central venous catheter (CVC)-related infections are commonly caused by Staphylococcus epidermidis that is able to form a biofilm on the catheter surface. Many studies involving biofilm formation by Staphylococcus have been published each adopting an own in vitro model. Since the capacity to form a biofilm depends on multiple environmental factors, direct comparison of results obtained in different studies remains challenging. This study characterized the phenotype (strong versus weak biofilm-producers) of S. epidermidis from CVCs in four different in vitro biofilm models, covering differences in material type (glass versus polymer) and nutrient presentation (static versus continuous flow). A good correlation in phenotype was obtained between glass and polymeric surfaces independent of nutrient flow, with 85% correspondence under static growth conditions and 80% under dynamic conditions. A 80% correspondence between static and dynamic conditions on polymeric surfaces could be demonstrated as well. Incubation time had a significant influence on the biofilm phenotype with only 55% correspondence between the dynamic models at different incubation times (48 h versus 17 h). Screening for the presence of biofilm-related genes only revealed that ica A was correlated with biofilm formation under static but not under dynamic conditions. In conclusion, this study highlights that a high level of standardization is necessary to interpret and compare results of different in vitro biofilm models.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/aa1bcd/133737.pdf
E-info
https://repository.uantwerpen.be/docman/iruaauth/6c3042/133737.pdf
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