Title
Human granulosa cells after ovulation induction show caspase-independent cell death Human granulosa cells after ovulation induction show caspase-independent cell death
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Basel ,
Subject
Human medicine
Source (journal)
Gynecologic and obstetric investigation. - Basel
Volume/pages
56(2003) :2 , p. 106-112
ISSN
0378-7346
ISI
000185390000010
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
It has become clear that apoptosis is an essential part of normal folliculogenesis and that granulosa cells in particular demonstrate intense cellular activity as well as programmed cell death. Although the entire mechanism of apoptosis appears to be conserved through many species, it now becomes clear that different cells may use different pathways within this system. We examined human granulosa cells after ovulation induction during an in vitro fertilization procedure to study apoptosis in this particular situation. We demonstrated a loss of cytokeratin staining as granulosa cells differentiate. We also detected that granulosa cells with apoptotic morphology did not stain for terminal deoxy-UTP nick end labeling and we showed the absence of immunoreactivity for caspase-cleaved cytokeratin, caspase-cleaved poly-ADP-ribose polymerase and caspase-cleaved caspase-3 in apoptotic granulosa cells. These data provide strong arguments for a caspase-independent cell death mechanism in human granulosa-lutein cells. Copyright (C) 2003 S. Karger AG, Basel.
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