Title
BiDiFuse : a FIJI plugin for fusing bi-directionally recorded microscopic image volumes
Author
Faculty/Department
Faculty of Sciences. Physics
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Oxford ,
Subject
Mathematics
Chemistry
Biology
Veterinary medicine
Engineering sciences. Technology
Computer. Automation
Source (journal)
Bioinformatics. - Oxford
Volume/pages
32(2016) :23 , p. 3691-3693
ISSN
1367-4803
1367-4803
ISI
000392749500026
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Deep tissue imaging is increasingly used for non-destructive interrogation of intact organs and small model organisms. An intuitive approach to increase the imaging depth by almost a factor of 2 is to record a sample from two sides and fuse both image stacks. However, imperfect three-dimensional alignment of both stacks presents a computational challenge. We have developed a FIJI plugin, called BiDiFuse, which merges bi-directionally recorded image stacks via 3D rigid transformations. The method is broadly applicable, considering its compatibility with all optical sectioning microscopes and does not rely on fiducial markers for image registration.
E-info
https://repository.uantwerpen.be/docman/iruaauth/80dc8f/134771.pdf
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Handle