Title
Detection of inflammatory cell function using <tex>$^{13}C$</tex> magnetic resonance spectroscopy of hyperpolarized [6-<tex>$^{13}C$</tex>]-arginine Detection of inflammatory cell function using <tex>$^{13}C$</tex> magnetic resonance spectroscopy of hyperpolarized [6-<tex>$^{13}C$</tex>]-arginine
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
London :Nature Publishing Group ,
Subject
Engineering sciences. Technology
Source (journal)
Scientific reports. - London, 2011, currens
Volume/pages
6(2016) , 10 p.
ISSN
2045-2322
2045-2322
Article Reference
31397
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Myeloid-derived suppressor cells (MDSCs) are highly prevalent inflammatory cells that play a key role in tumor development and are considered therapeutic targets. MDSCs promote tumor growth by blocking T-cell-mediated anti-tumoral immune response through depletion of arginine that is essential for T-cell proliferation. To deplete arginine, MDSCs express high levels of arginase, which catalyzes the breakdown of arginine into urea and ornithine. Here, we developed a new hyperpolarized C-13 probe, [6-C-13]-arginine, to image arginase activity. We show that [6-C-13]-arginine can be hyperpolarized, and hyperpolarized [C-13]-urea production from [6-C-13-arginine is linearly correlated with arginase concentration in vitro. Furthermore we show that we can detect a statistically significant increase in hyperpolarized [C-13]-urea production in MDSCs when compared to control bone marrow cells. This increase was associated with an increase in intracellular arginase concentration detected using a spectrophotometric assay. Hyperpolarized [6-C-13-arginine could therefore serve to image tumoral MDSC function and more broadly M2-like macrophages.
E-info
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Full text (open access)
https://repository.uantwerpen.be/docman/irua/319454/135014.pdf
Handle