Title
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Detection of inflammatory cell function using magnetic resonance spectroscopy of hyperpolarized [6-]-arginine
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Author
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Abstract
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Myeloid-derived suppressor cells (MDSCs) are highly prevalent inflammatory cells that play a key role in tumor development and are considered therapeutic targets. MDSCs promote tumor growth by blocking T-cell-mediated anti-tumoral immune response through depletion of arginine that is essential for T-cell proliferation. To deplete arginine, MDSCs express high levels of arginase, which catalyzes the breakdown of arginine into urea and ornithine. Here, we developed a new hyperpolarized C-13 probe, [6-C-13]-arginine, to image arginase activity. We show that [6-C-13]-arginine can be hyperpolarized, and hyperpolarized [C-13]-urea production from [6-C-13-arginine is linearly correlated with arginase concentration in vitro. Furthermore we show that we can detect a statistically significant increase in hyperpolarized [C-13]-urea production in MDSCs when compared to control bone marrow cells. This increase was associated with an increase in intracellular arginase concentration detected using a spectrophotometric assay. Hyperpolarized [6-C-13-arginine could therefore serve to image tumoral MDSC function and more broadly M2-like macrophages. |
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Language
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English
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Source (journal)
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Scientific reports. - London, 2011, currens
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Publication
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London
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Nature Publishing Group
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2016
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ISSN
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2045-2322
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DOI
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10.1038/SREP31397
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Volume/pages
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6
(2016)
, 10 p.
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Article Reference
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31397
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ISI
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000381133100001
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Pubmed ID
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27507680
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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