Publication
Title
Detection of inflammatory cell function using magnetic resonance spectroscopy of hyperpolarized [6-]-arginine
Author
Abstract
Myeloid-derived suppressor cells (MDSCs) are highly prevalent inflammatory cells that play a key role in tumor development and are considered therapeutic targets. MDSCs promote tumor growth by blocking T-cell-mediated anti-tumoral immune response through depletion of arginine that is essential for T-cell proliferation. To deplete arginine, MDSCs express high levels of arginase, which catalyzes the breakdown of arginine into urea and ornithine. Here, we developed a new hyperpolarized C-13 probe, [6-C-13]-arginine, to image arginase activity. We show that [6-C-13]-arginine can be hyperpolarized, and hyperpolarized [C-13]-urea production from [6-C-13-arginine is linearly correlated with arginase concentration in vitro. Furthermore we show that we can detect a statistically significant increase in hyperpolarized [C-13]-urea production in MDSCs when compared to control bone marrow cells. This increase was associated with an increase in intracellular arginase concentration detected using a spectrophotometric assay. Hyperpolarized [6-C-13-arginine could therefore serve to image tumoral MDSC function and more broadly M2-like macrophages.
Language
English
Source (journal)
Scientific reports. - London, 2011, currens
Publication
London : Nature Publishing Group, 2016
ISSN
2045-2322
Volume/pages
6(2016), 10 p.
Article Reference
31397
ISI
000381133100001
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 02.09.2016
Last edited 07.12.2017
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