Mutations in glucocerebrosidase are a major genetic risk factor for Parkinsons disease and increase susceptibility to dementia in a Flanders-Belgian cohort
Van Broeckhoven, Christine
Faculty of Medicine and Health Sciences
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Neuroscience letters. - Amsterdam
, p. 160-164
University of Antwerp
Objective To investigate the frequency of glucocerebrosidase (GBA) mutations in a Flanders-Belgian Parkinsons disease (PD) patient cohort and to assess genotype-phenotype correlations. Methods We performed an in-depth sequencing of all coding exons of GBA in 266 clinically well-characterized PD patients and 536 healthy control individuals. Results We identified rare, heterozygous GBA mutations in 12 PD patients (4.5%) and in 2 healthy control individuals (0.37%), confirming the genetic association of GBA mutations with PD in the Flanders-Belgian population (p < 0.001). The patient carriers had a more severe Unified Parkinsons Disease Rating Scale (UPDRS) motor score than non-carriers. Also, GBA mutation status was a significant, independent predictor for the presence of dementia (OR = 12.43, 95% CI: 2.2768.14. p = 0.004). Genetic association of PD with the common p.E326K and p.T369M variants in GBA was absent. Conclusion In our Flanders-Belgian cohort, carrier status of a heterozygous GBA mutation was a strong genetic risk factor for PD. The GBA mutation frequency of 4.5% is comparable to previously reported data in other European PD patient cohorts. Furthermore, our clinical data suggest a more severe motor phenotype and a strong predisposition to dementia in GBA mutation carriers.