Title
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Flow cytometric analysis of granulosa cells from follicular fluid after follicular stimulation
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Author
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Abstract
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Granulosa cells are not easily accessible, unless they are examined in follicular fluid after oocyte retrieval. These samples are usually contaminated with blood. We have set up a general technique for analysis of granulosa cells without physically separating them from blood cells. The sample is stained with CD45, which is a pan-leukocyte marker, and granulosa cells are consecutively selected as CD45 negative during flow cytometric analysis. Analysis of forward scatter of the granulosa cells, which is correlated to cell size, shows a wide size range throughout the whole population rather than two distinct populations as previously suggested. |
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Language
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English
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Source (journal)
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Human reproduction. - Bonn
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Publication
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Bonn
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1996
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ISSN
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0268-1161
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Volume/pages
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11
:10
(1996)
, p. 2211-2214
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Full text (publisher's version - intranet only)
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