Publication
Title
Confounding factors influencing amyloid beta concentration in cerebrospinal fluid
Author
Abstract
Background. Patients afflicted with Alzheimer's disease (AD) exhibit a decrease in the cerebrospinal fluid (CSF) concentration of the 42 amino acid form of -amyloid ( ). However, a high discrepancy between different centers in measured levels reduces the utility of this biomarker as a diagnostic tool and in monitoring the effect of disease modifying drugs. Preanalytical and analytical confounding factors were examined with respect to their effect on the measured level. Methods. Aliquots of CSF samples were either treated differently prior to measurement or analyzed using different commercially available xMAP or ELISA assays. Results. Confounding factors affecting CSF levels were storage in different types of test tubes, dilution with detergent-containing buffer, plasma contamination, heat treatment, and the origin of the immunoassays used for quantification. Conclusion. In order to conduct multicenter studies, a standardized protocol to minimize preanalytical and analytical confounding factors is warranted.
Language
English
Source (journal)
International journal of Alzheimer's disease. - Cairo, 2009, currens
Publication
Cairo : Hindawi Publishing Corporation , 2010
ISSN
2090-8024 [print]
2090-0252 [online]
DOI
10.4061/2010/986310
Volume/pages
(2010) , p. 1-11
Article Reference
986310
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
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Creation 17.11.2016
Last edited 22.08.2023
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