Title
Sequential determination of a combined <tex>$\gamma/\beta$</tex> and pure <tex>$\beta$</tex>-emitter by gamma and liquid scintillation counting: application to the transport of metals across fish gills Sequential determination of a combined <tex>$\gamma/\beta$</tex> and pure <tex>$\beta$</tex>-emitter by gamma and liquid scintillation counting: application to the transport of metals across fish gills
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York, N.Y. ,
Subject
Chemistry
Biology
Source (journal)
Analytical biochemistry. - New York, N.Y.
Volume/pages
224(1995) :1 , p. 92-99
ISSN
0003-2697
ISI
A1995QB30600012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
A combined gamma scintillation/liquid scintillation technique for the sequential determination of two radioactive tracers is described. The method was developed using a combined gamma/beta-emitter (Co-57) and a pure beta-emitter (Ca-45). First, the Co-57 radioactivity was determined by counting the samples in a gamma scintillation analyzer in the energy region 80-165 keV. Next, the samples were counted in a liquid scintillation analyzer. Only one energy region was used to count both isotopes to maximize the counting efficiencies. From the difference between quenched and unquenched beta-spectra, the counting region was set from 0 to 256 keV. The counting efficiency was related to a quench-indicating parameter (tSIE) for both nuclides by fitting a rectangular hyperbola to the quench data. By subtracting the Co-57 counts from the observed counts in the total window, Ca-45 dpm values were obtained. It is shown that the method presented gives reliable and consistent results. The recoveries of both isotopes are independent of the quench level in a large tSIE range, although five times more radioactivity is required for Ca-45 than for Co-57 to obtain accurate and reproducible results. The method has been used to study mechanisms of metal transport across biological interfaces. (C) 1995 Academic Press, Inc.
E-info
https://repository.uantwerpen.be/docman/iruaauth/d51079/fcc2983.pdf
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