Title Sequential determination of a combined $\gamma/\beta$ and pure $\beta$-emitter by gamma and liquid scintillation counting: application to the transport of metals across fish gillsSequential determination of a combined $\gamma/\beta$ and pure $\beta$-emitter by gamma and liquid scintillation counting: application to the transport of metals across fish gills Author van Ginneken, Luc Blust, Ronny Faculty/Department Faculty of Sciences. Biology Research group Systemic Physiological and Ecotoxicological Research (SPHERE) Department of Biology Publication type article Publication 1995New York, N.Y., 1995 Subject Chemistry Biology Source (journal) Analytical biochemistry. - New York, N.Y. Volume/pages 224(1995):1, p. 92-99 ISSN 0003-2697 ISI A1995QB30600012 Carrier E Target language English (eng) Full text (Publishers DOI) Affiliation University of Antwerp Abstract A combined gamma scintillation/liquid scintillation technique for the sequential determination of two radioactive tracers is described. The method was developed using a combined gamma/beta-emitter (Co-57) and a pure beta-emitter (Ca-45). First, the Co-57 radioactivity was determined by counting the samples in a gamma scintillation analyzer in the energy region 80-165 keV. Next, the samples were counted in a liquid scintillation analyzer. Only one energy region was used to count both isotopes to maximize the counting efficiencies. From the difference between quenched and unquenched beta-spectra, the counting region was set from 0 to 256 keV. The counting efficiency was related to a quench-indicating parameter (tSIE) for both nuclides by fitting a rectangular hyperbola to the quench data. By subtracting the Co-57 counts from the observed counts in the total window, Ca-45 dpm values were obtained. It is shown that the method presented gives reliable and consistent results. The recoveries of both isotopes are independent of the quench level in a large tSIE range, although five times more radioactivity is required for Ca-45 than for Co-57 to obtain accurate and reproducible results. The method has been used to study mechanisms of metal transport across biological interfaces. (C) 1995 Academic Press, Inc. E-info https://repository.uantwerpen.be/docman/iruaauth/d51079/fcc2983.pdf http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1995QB30600012&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1995QB30600012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1995QB30600012&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 Handle