Publication
Title
A framework to account for sedimentation and diffusion in particle-cell interactions
Author
Abstract
In vitro experiments provide a solid basis for understanding the interactions between particles and biological systems. An important confounding variable for these studies is the difference between the amount of particles administered and that which reaches the surface of cells. Here, we engineer a hydrogel-based nanoparticle system and combine in situ characterization techniques, 3D-printed cell cultures, and computational modeling to evaluate and study particle cell interactions of advanced particle systems. The framework presented demonstrates how sedimentation and diffusion can explain differences in particle cell association, and provides a means to account for these effects. Finally, using in silico modeling, we predict the proportion of particles that reaches the cell surface using common experimental conditions for a wide range of inorganic and organic micro- and nanoparticles. This work can assist in the understanding and control of sedimentation and diffusion when investigating cellular interactions of engineered particles.
Language
English
Source (journal)
Langmuir: the ACS journal of surfaces and colloids. - Washington, D.C.
Publication
Washington, D.C. : 2016
ISSN
0743-7463
DOI
10.1021/ACS.LANGMUIR.6B01634
Volume/pages
32 :47 (2016) , p. 12394-12402
ISI
000389117600017
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 10.01.2017
Last edited 09.10.2023
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