Publication
Title
Extended spectrum β-lactamase producing Escherichia coli in broiler breeding roosters : presence in the reproductive tract and effect on sperm motility
Author
Abstract
Extended spectrum beta-lactamases (ESBL)-producing Escherichia coli have emerged world-wide in animal husbandry and they were reported from different ecosystems. The purpose of this study was firstly, to investigate the presence of ESBL-producing E. calf in the gastrointestinal (GIT) and reproductive (RI) tracts of broiler breeding roosters, and secondly to study the impact of an ESBL-producing E. coli on artificially infected semen. A total of seventeen ESBL-producing E. coli strains were isolated from the gastrointestinal and reproductive tracts of nine broiler breeding roosters. All isolates were identified to the species level by API 20E system and MALDI-TOF, serotyped, and genetically characterized for ESBL production. Semen was artificially infected with E. coli ATCC25922 or with an ESBL-producing E. coli strain recovered from the reproductive tract. A computer aided semen analyzer (CASA) was used to compare different spermatozoa motility parameters in each sample. All ESBL-producing E. coli isolates could not be typed with the currently used sera and they were harboring a bla(CTX-M) gene alone or in combination with a bla(TEM) gene. The semen quality was notably less affected in samples infected with ESBL-producing E. coli strain compared to the control and sample infected with E. coli ATCC25922. The present study revealed that ESBL-producing E. coli can be isolated from both reproductive and digestive tracts of broiler breeding roosters. Contamination of the reproductive tract with ESBL-producing E. coli could lead to contamination of semen and could be an important factor in the dissemination of ESBL-producing E. coli in poultry. (C) 2015 Elsevier B.V. All rights reserved.
Language
English
Source (journal)
Animal reproduction science. - Amsterdam
Publication
Amsterdam : 2015
ISSN
0378-4320
DOI
10.1016/J.ANIREPROSCI.2015.06.021
Volume/pages
159 (2015) , p. 205-211
ISI
000358808800028
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identifier
Creation 31.01.2017
Last edited 28.01.2023
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