Title
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How to handle adsorption of cerebrospinal fluid amyloid-β (142) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ/Aβ ratio
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Author
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Abstract
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We aimed to investigate factors defining amyloid-β (142) (Aβ142) adsorption during preanalytical workup of cerebrospinal fluid (CSF). Methods CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ142 concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and IL-1RAcP. An enquiry assessed the number of transfers in current practice. Results In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5% loss of Aβ142 concentration, even 10% in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ140 was similar, resulting in stable Aβ142/Aβ140 ratios over multiple tube transfers. |
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Language
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English
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Source (journal)
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Alzheimer's & dementia / Alzheimer’s Association [Chicago, Ill.] - Orlando, Fla, 2005, currens
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Publication
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Orlando, Fla
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Elsevier
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2017
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ISSN
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1552-5260
[print]
1552-5279
[online]
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DOI
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10.1016/J.JALZ.2017.01.010
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Volume/pages
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13
:8
(2017)
, p. 885-892
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ISI
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000407041800005
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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