Publication
Title
How to handle adsorption of cerebrospinal fluid amyloid-β (142) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ/Aβ ratio
Author
Abstract
We aimed to investigate factors defining amyloid-β (142) (Aβ142) adsorption during preanalytical workup of cerebrospinal fluid (CSF). Methods CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ142 concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and IL-1RAcP. An enquiry assessed the number of transfers in current practice. Results In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5% loss of Aβ142 concentration, even 10% in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ140 was similar, resulting in stable Aβ142/Aβ140 ratios over multiple tube transfers.
Language
English
Source (journal)
Alzheimer's & dementia / Alzheimer’s Association [Chicago, Ill.] - Orlando, Fla, 2005, currens
Publication
Orlando, Fla : Elsevier , 2017
ISSN
1552-5260 [print]
1552-5279 [online]
DOI
10.1016/J.JALZ.2017.01.010
Volume/pages
13 :8 (2017) , p. 885-892
ISI
000407041800005
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 27.02.2017
Last edited 09.10.2023
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