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Title
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Influence of the incorporation of 1-(2,3-dideoxy-beta-d-erythro-hexopyranosyl)thymine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides
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Author
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Abstract
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| 1-(2,3-dideoxy-beta-D-erythro-hexopyranosyl)thymine was used at several positions as nucleoside substitute in the synthesis of oligonucleotides using the H-phosphonate chemistry. A 13-mer with one hexose nucleoside substitution at each end had equivalent hybridization properties as compared to the non-modified 13-mer. However, addition of a second analogue at each end or modification in the middle of a 13-mer caused a sharp decrease in melting temperature. Substitution at the 3'-end with two nucleoside analogues was necessary to withstand the action of snake venom phosphodiesterase. |
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Language
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English
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Source (journal)
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Bulletin des sociétés chimiques belges. - Gent, 1945, currens
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Publication
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Gent
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Rijksuniversiteit Gent
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1992
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ISSN
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0037-9646
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Volume/pages
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101
:2
(1992)
, p. 119-130
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ISI
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A1992HG10100006
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