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Title
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Developmental age and UV-B exposure co-determine antioxidant capacity and flavonol accumulation in Arabidopsis leaves
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Author
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Abstract
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| Developmental age is an important determinant of plant stress responses. In this study the importance of within-individual-heterogeneity of developmental age for plant UV-B responses was quantified. Arabidopsis thaliana rosettes were raised under indoor conditions, and the responses of leaves at different developmental stages were compared following exposure to supplemental UV-B radiation. Exposure to a low dose of UV-B had positive effects on concentrations of UV-absorbing pigments, quercetins and kaempferols as well as total antioxidant activity measured. Unlike UV-B, developmental age had a substantial effect on photochemistry, and especially energy dissipation. Younger leaves display relatively strong regulated dissipation, while older leaves show more non-regulated, non-photochemical energy dissipation. Developmental age also impacted on concentrations of UV-absorbing compounds, and antioxidant activity. In fact, developmental variation matched, or even exceeded the UV-induced response for these two parameters. Thus, pooling of rosette leaves is not necessarily a good strategy to visualise plant UV-responses. Rather, to fully understand plant UV-responses in a developmental context it is important to advance reporter technologies for physiological studies, including spin-trap technology to visualise in planta ROS and ROS-defences, and fluorescence excitation screening technology and chromogenic assays for in planta visualisation of specific UV-absorbing pigments. |
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Language
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English
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Source (journal)
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Environmental and experimental botany. - Oxford, 1976, currens
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Publication
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Oxford
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2017
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ISSN
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0098-8472
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DOI
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10.1016/J.ENVEXPBOT.2017.05.009
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Volume/pages
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140
(2017)
, p. 19-25
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ISI
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000405766900003
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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