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Diagnosis of aerobic vaginitis by quantitative real-time PCR
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Abstract
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Purpose To evaluate a real-time PCR-based technique to quantify bacteria associated with aerobic vaginitis (AV) as a potential test. Methods Vaginal samples from 100 women were tested by wet-mount microscopy, gram stain and quantitative real-time PCR targeting Enterobacteriacea, Staphylococcus spp., Streptococcus spp., Enterococcus spp., Escherichia coli, Streptococcus agalactiae, S. aureus; Lactobacillus spp. AV diagnosis obtained by wet-mount microscopy was used as reference. Results Some level of AV was diagnosed in 23 (23.7 %) cases. Various concentrations of Enterobacteriacea, Staphylococcus spp., Streptococcus spp. were detected an all patients. Enterococcus spp. were detected in 76 (78.3 %) cases. Summarized concentrations of aerobes were tenfold higher in AV-positive compared to AV-negative cases [7.30lg vs 6.06lg (p = 0.02)]. Concentrations of aerobes in severe, moderate and light AV cases did not vary significantly (p = 0.14). Concentration of lactobacilli was 1000-fold lower in AV-positive cases compared to normal cases (5.3lg vs 8.3lg, p < 0.0001). Streptococcus spp. dominated in the majority of AV-positive cases [19/22 (86.4 %) samples]. The relation of high loads of aerobes to the low numbers of Lactobacilli are a reliable marker for the presence of AV and could substitute microscopy as a test. Conclusions PCR may be a good standardized substitution for AV diagnosis in settings where well-trained microscopists are lacking. |
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Language
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English
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Source (journal)
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Archives of gynecology and obstetrics / Deutsche Gesellschaft für Gynäkologie und Geburtshilfe. - Berlin, 1987, currens
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Publication
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Berlin
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Springer
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2016
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ISSN
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0932-0067
[print]
1432-0711
[online]
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DOI
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10.1007/S00404-015-4007-4
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Volume/pages
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294
:1
(2016)
, p. 109-114
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ISI
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000378563400014
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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