Publication
Title
Bone engineering in dog mandible : coculturing mesenchymal stem cells with endothelial progenitor cells in a composite scaffold containing vascular endothelial growth factor
Author
Abstract
We sought to assess the effects of coculturing mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) in the repair of dog mandible bone defects. The cells were delivered in -tricalcium phosphate scaffolds coated with poly lactic co-glycolic acid microspheres that gradually release vascular endothelial growth factor (VEGF). The complete scaffold and five partial scaffolds were implanted in bilateral mandibular body defects in eight beagles. The scaffolds were examined histologically and morphometrically 8weeks after implantation. Histologic staining of the decalcified scaffolds demonstrated that bone formation was greatest in the VEGF/MSC scaffold (63.42 +/- 1.67), followed by the VEGF/MSC/EPC (47.8 +/- 1.87) and MSC/EPC (45.21 +/- 1.6) scaffolds, the MSC scaffold (34.59 +/- 1.49), the VEGF scaffold (20.03 +/- 1.29), and the untreated scaffold (7.24 +/- 0.08). Hence, the rate of new bone regeneration was highest in scaffolds containing MSC, either mixed with EPC or incorporating VEGF. Adding both EPC and VEGF with the MSC was not necessary. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1767-1777, 2017.
Language
English
Source (journal)
Journal of biomedical materials research: part B : applied biomaterials. - New York
Publication
New York : 2017
ISSN
1552-4973 [print]
1552-4981 [online]
DOI
10.1002/JBM.B.33707
Volume/pages
105 :7 (2017) , p. 1767-1777
ISI
000409231400004
Pubmed ID
27186846
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Project info
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 03.10.2017
Last edited 04.03.2024
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