|
Title
|
|
|
|
Nanometric distance measurements between Mn(II)DOTA centers
|
|
|
Author
|
|
|
|
|
|
|
Abstract
|
|
|
|
| Pulse electronelectron double resonance (PELDOR) is a versatile technique for probing the structures and functions of complex biological systems. Despite the recent interest in high-spin metal-ions for high field/frequency applications, PELDOR measurements of Mn(II) remain relatively underexplored. Here we present Mn(II)Mn(II) PELDOR distance measurements at 94 GHz on polyproline II (PPII) helices doubly spin-labeled with Mn(II)DOTA, which are distinguished by their small zero-field interaction. The measured MnMn distances and distribution profiles were in good agreement with the expected values from molecular models. Additional features in the frequency-domain spectra became apparent at certain combinations of detect and pump frequencies. Spin-Hamiltonian calculations showed that they likely arose from contributions from the pseudo-secular component of the dipolar interaction that were found to be non-negligible for Mn(II)DOTA. However, the influence of the pseudo-secular component on the distance distribution profiles apparently was limited. The results show the potential of Mn(II)DOTA spin labels for high-field PELDOR distance measurements in proteins and other biological systems. |
|
|
|
Language
|
|
|
|
Dutch
|
|
|
Source (journal)
|
|
|
|
Physical chemistry, chemical physics / Royal Society of Chemistry [London] - Cambridge, 1999, currens
|
|
|
Publication
|
|
|
|
Cambridge
:
The Royal Society of Chemistry
,
2015
|
|
|
ISSN
|
|
|
|
1463-9076
[print]
1463-9084
[online]
|
|
|
DOI
|
|
|
|
10.1039/C5CP03487F
|
|
|
Volume/pages
|
|
|
|
17
(2015)
, p. 23368-23377
|
|
|
ISI
|
|
|
|
000361142200009
|
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
|
Full text (publisher's version - intranet only)
|
|
|
|
|
|