Title
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Lysogenic conversion and phage resistance development in phage exposed Escherichia coli biofilms
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Author
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Abstract
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In this study, three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of infectious H-19B particles. On the other hand, a single dose of T7 rapidly led to resistance development in the biofilm population. Together, our data indicates a profound impact of phages on the dynamics within structured bacterial populations |
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Language
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English
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Source (journal)
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Viruses
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Publication
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2013
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ISSN
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1999-4915
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DOI
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10.3390/V5010150
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Volume/pages
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5
:1
(2013)
, p. 150-161
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Full text (Publisher's DOI)
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Full text (open access)
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