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Title
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Comparative evaluation of Broth microdilution with polystyrene and glass-coated plates, agar dilution, E-test, vitek, and disk diffusion for susceptibility testing of colistin and polymyxin B on carbapenem-resistant clinical isolates of acinetobacter baumannii
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Author
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Abstract
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| Introduction: With the increasing threat of multidrug-resistant organisms, such as Acinetobacter baumannii, the polymyxin class of drugs (colistin and polymyxin B) has become popular in clinical practice. A better understanding of antimicrobial susceptibility testing methods for colistin and polymyxin B is needed for optimal patient management. Materials and Methods: Forty-two carbapenem-resistant A. baumannii isolates were subjected to susceptibility testing for colistin and polymyxin B using the following methods: broth microdilution (BMD) (glass-coated plates [BMD-Gs] and polystyrene plates [BMD-Ps]), agar dilution (AD), E-test((R)), Vitek((R)), and disk diffusion. Using BMD as the gold standard, comparative analysis between different methods was carried out. Results: With BMD-Gs as reference, reliability was high for BMD-Ps and moderate for AD and Vitek for both the drugs. Similar results were obtained when the BMD-P was used as reference, but drug-polystyrene interaction was observed. Conclusion: Different susceptibility testing methods for polymyxins show great variation in their results and BMD using glass-coated plates can be considered the best candidate for gold standard. |
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Language
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English
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Source (journal)
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Microbial drug resistance : mechanism, epidemiology, and disease .. - Larchmont, N.Y.
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Publication
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New rochelle
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Mary ann liebert, inc
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2018
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ISSN
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1076-6294
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DOI
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10.1089/MDR.2017.0251
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Volume/pages
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7 p.
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ISI
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000424226300001
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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