Publication
Title
Interleukin-15-cultered dendritic cells enhance anti-tumor gamme delta T cell functions through IL-15 secretion
Author
Abstract
Dendritic cell (DC) vaccination can be an effective post-remission therapy for acute myeloid leukemia (AML). Yet, current DC vaccines do not encompass the ideal stimulatory triggers for innate gamma delta (gamma delta) T cell anti-tumor activity. Promoting type 1 cytotoxic gamma delta T cells in patients with AML is, however, most interesting, considering these unconventional T cells are primed for rapid function and exert meaningful control over AML. In this work, we demonstrate that interleukin (IL)-15 DCs have the capacity to enhance the anti-tumoral functions of gamma delta T cells. IL-15 DCs of healthy donors and of AML patients in remission induce the upregulation of cytotoxicity-associated and co-stimulatory molecules on the gamma delta T cell surface, but not of co-inhibitory molecules, incite gamma delta T cell proliferation and stimulate their interferon-gamma production in the presence of blood cancer cells and phosphoantigens. Moreover, the innate cytotoxic capacity of gamma delta T cells is significantly enhanced upon interaction with IL-15 DCs, both towards leukemic cell lines and allogeneic primary AML blasts. Finally, we address soluble IL-15 secreted by IL-15 DCs as the main mechanism behind the IL-15 DC-mediated gamma delta T cell activation. These results indicate that the application of IL-15-secreting DC subsets could render DC-based anti-cancer vaccines more effective through, among others, the involvement of gamma delta T cells in the anti-leukemic immune response.
Language
English
Source (journal)
Frontiers in immunology. - Place of publication unknown
Publication
Place of publication unknown : 2018
ISSN
1664-3224
Volume/pages
9(2018), 12 p.
Article Reference
658
ISI
000429581700001
Pubmed ID
29692776
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
CD56+ human blood dendritic cells: unique mediators of strong natural killer and gamma-delta T cell activation to promote antigen-specific T cell immune responses in health and disease.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 04.05.2018
Last edited 02.08.2021
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