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Title
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A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
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Author
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Abstract
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| There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resinbased dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva with ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), using an extraction step and additional derivatization of BPA with pyridine-3-sulfonyl chloride. Light-cured resin-based composites were incubated at 37 degrees C in 1 mL artificial saliva, which was refreshed daily for one week. The final protocol allows accurate quantification of very low levels of BPA in samples of artificial saliva (i.e. 1.10 pmol BPA/mL or 250 pg/mL). The daily BPA-release from dental composites, ranging from 1.10 to 7.46 pmol BPA/mL, was characterized over a period of 7 days. The highest total amount of BPA was released from Solitaire 2 (24.72 +/- 2.86 pmol), followed by G-aenial Posterior (15.51 +/- 0.88 pmol) and Filtek Supreme XTE (12.00 +/- 1.31 pmol). In contrast, only trace amounts of BPA were released from Ceram. x Universal. This UPLC-MS/MS method might be used for clinical research focusing on the evaluation of the clinical relevance of BPA release from dental materials. |
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Language
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English
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Source (journal)
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Scientific reports. - London, 2011, currens
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Publication
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London
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Nature Publishing Group
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2018
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ISSN
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2045-2322
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DOI
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10.1038/S41598-018-24815-Z
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Volume/pages
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8
(2018)
, 7 p.
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Article Reference
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6981
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ISI
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000431291500033
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Pubmed ID
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29725047
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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