Publication
Title
Estimation of Rates of Reactions Triggered by Electron Transfer in Top-Down Mass Spectrometry
Author
Abstract
Electron transfer dissociation (ETD) is a versatile technique used in mass spectrometry for the high-throughput characterization of proteins. It consists of several competing reactions triggered by the transfer of an electron from its anion source to the sample cations. One can retrieve relative quantities of the products from mass spectra. We present a method to analyze these results from the perspective of the reaction kinetics. A formal mathematical model of the ETD process is introduced and parametrized by intensities of the occurring reactions. Also, we introduce a method to estimate the reaction intensities by solving a nonlinear optimization problem. The presented method proves highly robust to noise on in silico generated data. Moreover, the presented model can explain a considerable amount of experimental results obtained under various experimental settings.
Language
English
Source (journal)
Lecture notes in computer science. - Berlin, 1973, currens
Source (book)
13th International Symposium on Bioinformatics Research and Applications, (ISBRA), MAY 29-JUN 02, 2017, Honolulu, HI
Publication
Cham : Springer international publishing ag, 2017
ISBN
978-3-319-59574-0
978-3-319-59575-7
978-3-319-59574-0
Volume/pages
10330(2017), p. 96-107
ISI
000434328000009
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Project info
An integrated informatics platform for mass spectrometry-based protein assays (InSPECtor).
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 10.07.2018
Last edited 24.08.2021
To cite this reference