Publication
Title
CBL1-CIPK26 mediated phosphorylation enhances activity of the NADPH oxidase RBOHC, but is dispensable for root hair growth
Author
Abstract
Root hairs (RH) are tip growing polarized cells aiding the uptake of nutrients and water into plants. RH differentiation involves the interplay of various hormones and second messengers. Tightly regulated production of reactive oxygen species by the NADPH oxidase RBOHC crucially functions in RH differentiation and Ca2+‐dependent phosphorylation has been implemented in these processes. However, the kinases regulating RBOHC remained enigmatic. Here we identify CBL1‐CIPK26 Ca2+ sensor‐kinase complexes as modulators of RBOHC activity. Combined genetic, cell biological and biochemical analyzes reveal synergistic function of CIPK26‐mediated phosphorylation and Ca2+ binding for RBOHC activation. Complementation of rbohC mutant RH phenotypes by a S318/322 phosphorylation deficient RBOHC version suggests flexible and alternating phosphorylation patterns as mechanism fine‐tuning ROS production in RH development.
Language
English
Source (journal)
FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
Publication
Amsterdam : 2018
ISSN
0014-5793
Volume/pages
592 :15 (2018) , p. 2582-2593
ISI
000441436400008
Pubmed ID
29992537
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
Growth and development of higher plants.
Regulation of cell elongation during Arabidopsis thaliana development.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 17.07.2018
Last edited 20.11.2021
To cite this reference