DNA adduct profiling of in vitro colonic meat digests to map red vs. white meat genotoxicity

Hemeryck, Lieselot Y.; Rombouts, Caroline; De Paepe, Ellen; Vanhaecke, Lynn

doi:10.1016/J.FCT.2018.02.032

Title

DNA adduct profiling of in vitro colonic meat digests to map red vs. white meat genotoxicity

Author

Hemeryck, Lieselot Y.

Rombouts, Caroline

De Paepe, Ellen

Vanhaecke, Lynn

Abstract

The consumption of red meat has been linked to an increased colorectal cancer (CRC) risk. One of the major hypotheses states that heme iron (present in red meat) stimulates the formation of genotoxic N-nitroso compounds (NOCs) and lipid peroxidation products (LPOs). By means of DNA adductomics, chemically induced DNA adduct formation can be mapped in relation to e.g. dietary exposures. In this study, this state-of-the-art methodology was used to investigate alkylation and (lipid per)oxidation induced DNA adduct formation in in vitro red vs. white meat digests. In doing so, 90 alkylation and (lipid per)oxidation induced DNA adduct types could be (tentatively) identified. Overall, 12 NOC- and/or LPO-related DNA adduct types, i.e. dimethyl-T (or ethyl-T), hydroxymethyl-T, tetramethyl-T, methylguanine (MeG), guanidinohydantoin, hydroxybutyl-C, hydroxymethylhydantoin, malondialdehyde-x3-C, O-6-carboxymethylguanine, hydroxyethyl-T, carboxyethyl-T and 3,N-4-etheno-C were singled out as potential heme-rich meat digestion markers. The retrieval of these DNA adduct markets is in support of the heme, NOC and LPO hypotheses, suggesting that DNA adduct formation may indeed contribute to red meat related CRC risk.

Language

English

Source (journal)

Food and chemical toxicology. - Oxford

Publication

Oxford : 2018

ISSN

0278-6915

DOI

10.1016/J.FCT.2018.02.032

Volume/pages

115 (2018) , p. 73-87