Publication
Title
Neuroimaging of subacute brain inflammation and microstructural changes predicts long-term functional outcome after experimental traumatic brain injury
Author
Abstract
There is currently a lack of prognostic biomarkers to predict the different sequelae following traumatic brain injury (TBI). The present study investigated the hypothesis that subacute neuroinflammation and microstructural changes correlate with chronic TBI deficits. Rats were subjected to Controlled Cortical Impact (CCI) injury, sham surgery or skin incision (naïve). CCI-injured (n=18) and sham-operated rats (n=6) underwent positron emission tomography (PET) imaging with the translocator protein (TSPO) radioligand [18F]PBR111 and diffusion tensor imaging (DTI) in the subacute phase (≤3 weeks post-injury) to quantify inflammation and microstructural alterations. CCI-injured, sham-operated and naïve rats (n=8) underwent behavioural testing in the chronic phase (5.5-10 months post-injury): open field and sucrose preference tests, two one-week video-EEG monitoring periods, pentylenetetrazole seizure susceptibility tests, and a Morris water maze test. In vivo imaging revealed pronounced neuroinflammation, decreased fractional anisotropy and increased diffusivity in perilesional cortex and ipsilesional hippocampus of CCI-injured rats. Behavioural analysis revealed disinhibition, anhedonia, increased seizure susceptibility and impaired learning in CCI-injured rats. Subacute TSPO expression and changes in DTI metrics significantly correlated with several chronic deficits (Pearsons r = 0.50-0.90). Certain specific PET and DTI parameters had good sensitivity and specificity (area under the ROC curve=0.85-1.00) to distinguish between TBI animals with and without particular behavioural deficits. Depending on the investigated behavioural deficit, PET or DTI data alone, or the combination, could very well predict the variability in functional outcome data (adjusted R2=0.54-1.00). Taken together, both TSPO PET and DTI seem promising prognostic biomarkers to predict different chronic TBI sequelae.
Language
English
Source (journal)
Journal of neurotrauma. - New York
Journal of neurotrauma. - New York
Publication
New York : 2018
ISSN
1557-9042 [online]
0897-7151 [print]
Volume/pages
35 (2018) , 21 p.
ISI
000446275000001
Pubmed ID
30032713
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
An integrated approach towards understanding the pathogenesis of neurodegeneration (NEUROBRAINNET).
NEURON II - Proteolytic remodeling of the extracellular matrix in aberrant synaptic plasticity underlying epilepsy evoked by traumatic brain injury (TBI Epilepsy).
The role of the extracellular matrix proteases MMP-9 and uPA in the development of posttraumatic epilepsy following traumatic brain injury.
The role of the extracellular matrix proteases MMP-9 and uPA in the development of posttraumatic epilepsy following traumatic brain injury.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 03.09.2018
Last edited 15.10.2021
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