Publication
Title
Effects of vitrification on the viability of alginate encapsulated isolated bovine pre-antral follicles
Author
Abstract
Purpose Individual follicle cryopreservation techniques, without hydrogel support, are labor-intensive and a substantial proportion of isolated follicles are lost during handling and after warming. Therefore, the viability and morphology of isolated bovine (as a model for human) pre-antral follicles after vitrification and warming, when encapsulated in alginate beads, were investigated. Methods Bovine pre-antral follicles were mechanically isolated and divided into four different groups: (1) culture in 2% alginate beads (3D system) and vitrification in beads usingmesh cups (3DVIT), (2) culture in 2% alginate beads (3DCUL), (3) culture in 96well plates (2D system) and vitrification using High Security Vitrification straws (R) (2DVIT), (4) culture in a 2D system (2DCUL). The same vitrification and warming protocols were used for embedded (3DVIT) and non-embedded follicles (2DVIT). Results No differences were observed in follicle viability between group 2DCUL and 3DCUL. Group 3DVITshowed the lowest viability (45.9%) according to calcein and neutral red staining among all groups. Group 2DVIT displayed the highest viability (87.5%) and largest percentage of follicles with a well-preserved morphology. Conclusions Our results show that, using a vitification protocol optimized for non-embedded follicles, 2D culture is more effective in vitrifying isolated follicles. However, embedding in alginate allow to handle follicles more efficiently, i. e., without excessive manipulation and thus less labor-intensive in combination with a reduced loss of follicles during the procedure. Based on the increased work efficiency, but lower viability and higher proportion of follicles showing impairedmorphology, we consider it advantageous to optimize the protocol for the vitrification of embedded follicles to increase survival and maintain morphology after vitrification.
Language
English
Source (journal)
Journal of assisted reproduction and genetics. - New York, N.Y.
Publication
New York, N.Y. : 2018
ISSN
1058-0468
Volume/pages
35 :7 (2018) , p. 1187-1199
ISI
000440113200009
Pubmed ID
29797286
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 07.09.2018
Last edited 20.09.2021
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