Publication
Title
Functionalization of gold nanoparticles with nanobodies through physical adsorption
Author
Abstract
Nanobodies (Nbs) are single-domain antibodies with highly desirable properties employed as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have a significant potential for the development of highly specific and user-friendly diagnostic assays. These immuno-assays often employ gold nanoparticles (AuNPs) as a detection label. Despite great advances in Nb technology and the immuno-sensor field, the functionalization of AuNPs with Nbs mostly remains unchartered territory. In this paper, we present a study of the functionalization of AuNPs with Nbs through physical adsorption. We show that Nb pI, solution pH, additives and protein engineering strategies have profound effects on the generation of stable Nb-AuNPs. Our results further demonstrate that physical adsorption can be used to produce functional Nb-AuNPs that could be employed in a wide array of applications.
Language
English
Source (journal)
Analytical methods / Royal Society of Chemistry [Londen] - Cambridge
Publication
Cambridge : 2017
ISSN
1759-9660
Volume/pages
9:23(2017), p. 3430-3440
ISI
000403558800005
Full text (Publisher's DOI)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 04.10.2018
Last edited 26.07.2021