Publication
Title
Substrate recognition and activity regulation of the escherichia coli mRNA endonuclease MazF
Author
Abstract
Escherichia coli MazF (EcMazF) is the archetype of a large family of ribonucleases involved in bacterial stress response. The crystal structure of EcMazF in complex with a 7-nucleotide substrate mimic explains the relaxed substrate specificity of the E. coli enzyme relative to its Bacillus subtilis counterpart and provides a framework for rationalizing specificity in this enzyme family. In contrast to a conserved mode of substrate recognition and a conserved active site, regulation of enzymatic activity by the antitoxin EcMazE diverges from its B. subtilis homolog. Central in this regulation is an EcMazE-induced double conformational change as follows: a rearrangement of a crucial active site loop and a relative rotation of the two monomers in the EcMazF dimer. Both are induced by the C-terminal residues Asp-78-Trp-82 of EcMazE, which are also responsible for strong negative cooperativity in EcMazE-EcMazF binding. This situation shows unexpected parallels to the regulation of the F-plasmid CcdB activity by CcdA and further supports a common ancestor despite the different activities of the MazF and CcdB toxins. In addition, we pinpoint the origin of the lack of activity of the E24A point mutant of EcMazF in its inability to support the substrate binding-competent conformation of EcMazF.
Language
English
Source (journal)
Journal of biological chemistry. - Baltimore, Md
Publication
Baltimore, Md : 2016
ISSN
0021-9258 [print]
1083-351X [online]
Volume/pages
291 :21 (2016) , p. 10950-10960
ISI
000377042100001
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Project info
BIOSTRUCT-X: Transnational access and enhancement of integrated Biological Structure determination at synchrotron X-ray radiation facilities
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 04.10.2018
Last edited 26.09.2021