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Validation of a simple, fast liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 40 antidepressant drugs or their metabolites in plasma
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| Abstract |
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| Introduction: Antidepressant (AD) use has increased significantly over the last decades. Therapeutic drug monitoring is recommended for compliance, toxicity and treatment efficiency. ADs also show a high prevalence in forensic cases. Few methods have been developed that combine a fast, easy sample clean-up with a quantification based on liquid chromatography-triple quadrupole mass spectrometry (LC-QQQ). Methodology: A liquid-liquid extraction (LLE) was performed using 200 mu L of plasma. The evaporated and reconstituted upper fraction was injected on a LC-QQQ system monitoring 3 transitions per compound. The method was fully validated according to international guidelines. Results & discussion: The chromatographic run time was under 12 min. The LLE was successful in removing interferences with minimal sensitivity loss. Calibration curves ranged from sub-therapeutic to toxic concentrations. Quality control samples showed high accuracy (81%-119%) and precision (<= 14%) within and between batches. Stability was tested at ambient temperature and - 20 degrees C. The method was successfully applied to external quality control and case samples. Conclusion: The presented method successfully quantifies 40 compounds of interest. Because of a simple sample clean-up, a relatively short chromatographic run and a wide calibration range this method can be implemented in therapeutic drug monitoring, forensic research and related fields. |
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English
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| Source (journal) |
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Clinica chimica acta. - Amsterdam |
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| Publication |
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Amsterdam : 2018
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0009-8981
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485(2018), p. 243-257
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| ISI |
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000447946700039
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29966618
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| Full text (publisher's version - intranet only) |
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