Publication
Title
Validation of a simple, fast liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 40 antidepressant drugs or their metabolites in plasma
Author
Abstract
Introduction: Antidepressant (AD) use has increased significantly over the last decades. Therapeutic drug monitoring is recommended for compliance, toxicity and treatment efficiency. ADs also show a high prevalence in forensic cases. Few methods have been developed that combine a fast, easy sample clean-up with a quantification based on liquid chromatography-triple quadrupole mass spectrometry (LC-QQQ). Methodology: A liquid-liquid extraction (LLE) was performed using 200 mu L of plasma. The evaporated and reconstituted upper fraction was injected on a LC-QQQ system monitoring 3 transitions per compound. The method was fully validated according to international guidelines. Results & discussion: The chromatographic run time was under 12 min. The LLE was successful in removing interferences with minimal sensitivity loss. Calibration curves ranged from sub-therapeutic to toxic concentrations. Quality control samples showed high accuracy (81%-119%) and precision (<= 14%) within and between batches. Stability was tested at ambient temperature and - 20 degrees C. The method was successfully applied to external quality control and case samples. Conclusion: The presented method successfully quantifies 40 compounds of interest. Because of a simple sample clean-up, a relatively short chromatographic run and a wide calibration range this method can be implemented in therapeutic drug monitoring, forensic research and related fields.
Language
English
Source (journal)
Clinica chimica acta. - Amsterdam
Publication
Amsterdam : 2018
ISSN
0009-8981
Volume/pages
485(2018), p. 243-257
ISI
000447946700039
Pubmed ID
29966618
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
The rise and threat of new psychoactive substances: integrated research to obtain evidence-based information on their properties and actual use.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 08.10.2018
Last edited 15.07.2021
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