|
Title
|
|
|
|
Validation of a simple, fast liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 40 antidepressant drugs or their metabolites in plasma
|
|
|
Author
|
|
|
|
|
|
|
Abstract
|
|
|
|
| Introduction: Antidepressant (AD) use has increased significantly over the last decades. Therapeutic drug monitoring is recommended for compliance, toxicity and treatment efficiency. ADs also show a high prevalence in forensic cases. Few methods have been developed that combine a fast, easy sample clean-up with a quantification based on liquid chromatography-triple quadrupole mass spectrometry (LC-QQQ). Methodology: A liquid-liquid extraction (LLE) was performed using 200 mu L of plasma. The evaporated and reconstituted upper fraction was injected on a LC-QQQ system monitoring 3 transitions per compound. The method was fully validated according to international guidelines. Results & discussion: The chromatographic run time was under 12 min. The LLE was successful in removing interferences with minimal sensitivity loss. Calibration curves ranged from sub-therapeutic to toxic concentrations. Quality control samples showed high accuracy (81%-119%) and precision (<= 14%) within and between batches. Stability was tested at ambient temperature and - 20 degrees C. The method was successfully applied to external quality control and case samples. Conclusion: The presented method successfully quantifies 40 compounds of interest. Because of a simple sample clean-up, a relatively short chromatographic run and a wide calibration range this method can be implemented in therapeutic drug monitoring, forensic research and related fields. |
|
|
|
Language
|
|
|
|
English
|
|
|
Source (journal)
|
|
|
|
Clinica chimica acta. - Amsterdam, 1956, currens
|
|
|
Publication
|
|
|
|
Amsterdam
:
2018
|
|
|
ISSN
|
|
|
|
0009-8981
[print]
1873-3492
[online]
|
|
|
DOI
|
|
|
|
10.1016/J.CCA.2018.06.047
|
|
|
Volume/pages
|
|
|
|
485
(2018)
, p. 243-257
|
|
|
ISI
|
|
|
|
000447946700039
|
|
|
Pubmed ID
|
|
|
|
29966618
|
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
|
Full text (publisher's version - intranet only)
|
|
|
|
|
|