Publication
Title
Protocol for acute and chronic ecotoxicity testing of the turquoise killifish nothobranchius furzeri
Author
Abstract
The killifish Nothobranchius furzeri is an emerging model organism in the field of ecotoxicology and its applicability in acute and chronic ecotoxicity testing has been demonstrated. Overall, the sensitivity of the species to toxic compounds is in the range with, or higher than, that of other model species. This work describes protocols for acute, chronic, and multigenerational bioassays of single and combined stressor effects on N. furzeri. Due to its short maturation time and life-cycle, this vertebrate model allows the study of endpoints such as maturation time and fecundity within four months. Transgenerational full life-cycle exposure trials can be performed in as little as 8 months. Since this species produces eggs that are drought-resistant and remain viable for years, the on-site culture of the species is not needed but individuals can be recruited when required. The protocols are designed to measure life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
Language
English
Source (journal)
Journal of visualized experiments. - -
Publication
2018
ISSN
1940-087X
DOI
10.3791/57308
Volume/pages
134 (2018) , 7 p.
Article Reference
e57308
ISI
000444051300073
Pubmed ID
29757283
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
Mixed metal and temperature stress in aquatic environments establishing functional links across different levels of organisation.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 08.10.2018
Last edited 02.10.2024
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