Publication
Title
Inflammatory mediators in Escherichia coli-induced mastitis in mice
Author
Abstract
Escherichia coli (E coli) infections in mouse mammary glands are rarely described and poorly characterized. In order to investigate the host immune response during coliform mastitis, several inflammatory parameters were evaluated at 24 and 48 h following inoculation of mouse mammary glands with E coli. Successfully challenged mice showed high values of the acute phase protein serum amyloid A (SAA) in blood. Systemic concentrations of the major inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were also increased as compared to control mice, while interleukin-1 (IL-1) levels remained negligible. Infected mammary glands showed a significant increase of all cytokine levels as compared to control glands. In accordance, mammary expression of the biologically inactive proform of IL-1 beta was strongly up-regulated. Remarkably, data obtained in wild type as well as caspase-1 knockout mice showed that IL-1 beta maturation seemed to occur independently from caspase-1. Finally, E coli infection also triggered activation of the nuclear transcription factor-kappa B (NF-kappa B) in the mammary gland. In conclusion, the current study provides novel insights on the contribution of major regulatory proteins to the acute inflammatory host response at the local and systemic level during E coli mastitis in mice. (c) 2007 Elsevier Ltd. All rights reserved.
Language
English
Source (journal)
Comparative immunology, microbiology and infectious diseases. - Oxford
Publication
Oxford : 2008
ISSN
0147-9571
Volume/pages
31 :6 (2008) , p. 551-565
ISI
000261669400010
Full text (Publisher's DOI)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 18.10.2018
Last edited 02.12.2021