Impact of primary mouse macrophage cell types on Leishmania infection and in vitro drug susceptibility
Primary mouse macrophages are frequently used to provide an in vitro intracellular model to evaluate antileishmanial drug efficacy. The present study compared the phenotypic characteristics of Swiss, BALB/c, and C57BL/6 mouse bone marrow-derived macrophages and peritoneal exudate cells using different stimulation and adherence protocols upon infection with a Leishmania infantum laboratory strain and two clinical isolates. Evaluation parameters were susceptibility to infection, permissiveness to amastigote multiplication, and impact on drug efficacy. Observed variations in infection of peritoneal exudate cells can mostly be linked to changes in the inflammatory cytokine profiles (IL-6, TNF-α, KC/GRO) rather than to differences in initial production of nitric oxide and reactive oxygen species. Optimization of the cell stimulation and adherence conditions resulted in comparable infection indices among peritoneal exudate cells and the various types of bone marrow-derived macrophages. BALB/c-derived bone marrow-derived macrophages were slightly more permissive to intracellular amastigote replication. Evaluation of antileishmanial drug potency in the various cell systems revealed minimal variation for antimonials and paromomycin, and no differences for miltefosine and amphotericin B. The study results allow to conclude that drug evaluation can be performed in all tested primary macrophages as only marginal differences are observed in terms of susceptibility to infection and impact of drug exposure. Combined with some practical considerations, the use of 24-h starch-stimulated, 48-h adhered, Swiss-derived peritoneal exudate cells can be advocated as an efficient, reliable, relatively quick, and cost-effective tool for routine drug susceptibility testing in vitro whenever the use of primary cells is feasible.
Source (journal)
Parasitology research / Deutsche Gesellschaft für Parasitologie. - Berlin, 1987, currens
Berlin : Springer , 2018
0932-0113 [print]
1432-1955 [online]
117 :11 (2018) , p. 3601-3612
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Project info
Veterinary and human parasitology.
Dynamics and mechanisms of paromomycin and miltefosine drug-resistance in the protozoan parasite Leishmania.
Identifying factors involved in miltefosine or amphotericin B treatment failure in visceral leishmaniasis.
The role of parasite sanctuary sites and interaction with Kupffer cells in treatment failure of Visceral Leishmaniasis.
Publication type
Publications with a UAntwerp address
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Web of Science
Creation 29.10.2018
Last edited 15.11.2022
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