Publication
Title
Radical solutions : principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure
Author
Abstract
'] In recent years, electron capture (ECD) and electron transfer dissociation (ETD) have emerged as two of the most useful methods in mass spectrometry-based protein analysis, evidenced by a considerable and growing body of literature. In large part, the interest in these methods is due to their ability to induce backbone fragmentation with very little disruption of noncovalent interactions which allows inference of information regarding higher order structure from the observed fragmentation behavior. Here, we review the evolution of electron-based dissociation methods, and pay particular attention to their application in "native" mass spectrometry, their mechanism, determinants of fragmentation behavior, and recent developments in available instrumentation. Although we focus on the two most widely used methodsECD and ETDwe also discuss the use of other ion/electron, ion/ion, and ion/neutral fragmentation methods, useful for interrogation of a range of classes of biomolecules in positive- and negative-ion mode, and speculate about how this exciting field might evolve in the coming years.
Language
English
Source (journal)
Mass spectrometry reviews. - New York, N.Y., 1982, currens
Publication
Hoboken : Wiley , 2018
ISSN
0277-7037
Volume/pages
37 :6 (2018) , p. 750-771
ISI
000446306400003
Pubmed ID
29425406
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 09.11.2018
Last edited 04.09.2021
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