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Title
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Transcriptome profiling in blood before and after hepatitis B vaccination shows significant differences in gene expression between responders and non-responders
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Author
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Abstract
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| Introduction: As the hepatitis B virus is widely spread and responsible for considerable morbidity and mortality, WHO recommends vaccination from infancy to reduce acute infection and chronic carriers. However, current subunit vaccines are not 100% efficacious and leave 5-10% of recipients unprotected. Methods: To evaluate immune responses after Engerix-B vaccination, we determined, using mRNA-sequencing, whole blood early gene expression signatures before, at day 3 and day 7 after the first dose and correlated this with the resulting antibody titer after two vaccine doses. Results: Our results indicate that immune related genes are differentially expressed in responders mostly at day 3 and in non-responders mostly at day 7. The most remarkable difference between responders and non-responders were the differentially expressed genes before vaccination. The granulin precursor gene (GRN) was significantly downregulated in responders while upregulated in non-responders at day 0. Furthermore, absolute granulocytes numbers were significantly higher in non-responders at day 0. Conclusion: The non-responders already showed an activated state of the immune system before vaccination. Furthermore, after vaccination, they exhibited a delayed and partial immune response in comparison to the responders. Our data may indicate that the baseline and untriggered immune system can influence the response upon hepatitis B vaccination. (C) 2018 Elsevier Ltd. All rights reserved. |
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Language
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English
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Source (journal)
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Vaccine / International Society for Vaccines. - Amsterdam
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Publication
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Amsterdam
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2018
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ISSN
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0264-410X
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DOI
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10.1016/J.VACCINE.2018.09.001
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Volume/pages
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36
:42
(2018)
, p. 6282-6289
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ISI
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000447479200005
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Pubmed ID
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30205979
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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