Publication
Title
Vibrational circular dichroism sheds new light on the competitive effects of crowding and $\beta$-synuclein on the fibrillation process of $\alpha$-synuclein
Author
Abstract
 The effects of crowding, using the crowding agent Ficoll 70, and the presence of beta-synuclein on the fibrillation process of a-synuclein were studied by spectroscopic techniques, transmission electron microscopy, and thioflavin T assays. This combined approach, in which all techniques were applied to the same original sample, generated an unprecedented understanding of the effects of these modifying agents on the morphological properties of the fibrils. Separately, crowding gives rise to shorter mutually aligned fibrils, while beta-synuclein leads to branched, short fibrils. The combination of both effects leads to short, branched, mutually aligned fibrils. Moreover, it is shown that the nondestructive technique of vibrational circular dichroism is extremely sensitive to the length and the higher order morphology of the fibrils.
Language
English
Source (journal)
Biochemistry / American Chemical Society. - Washington, D.C., 1962, currens
Publication
Washington, D.C. : 2018
ISSN
0006-2960 [print]
1520-4995 [online]
Volume/pages
57 :41 (2018) , p. 5989-5995
ISI
000447815900010
Pubmed ID
30239196
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
 Faculty/Department Research group Project info 4D Protein Structure. Publication type Subject Affiliation Publications with a UAntwerp address