Publication
Title
Plasma donor-derived cell-free DNA kinetics after kidney transplantation using a single tube multiplex PCR assay
Author
Abstract
Background After transplantation, cell-free DNA derived from the donor organ (ddcfDNA) can be detected in the recipients circulation. We aimed to quantify ddcfDNA levels in plasma of kidney transplant recipients thereby investigating the kinetics of this biomarker after transplantation and determining biological variables that influence ddcfDNA kinetics in stable and non-stable patients. Materials and methods From 107 kidney transplant recipients, plasma samples were collected longitudinally after transplantation (day 13 months) within a multicenter set-up. Cell-free DNA from the donor was quantified in plasma as a fraction of the total cell-free DNA by next generation sequencing using a targeted, multiplex PCR-based method for the analysis of single nucleotide polymorphisms. A subgroup of stable renal transplant recipients was identified to determine a ddcfDNA threshold value. Results In stable transplant recipients, plasma ddcfDNA% decreased to a mean (SD) ddcfDNA% of 0.46% (± 0.21%) which was reached 9.85 (± 5.6) days after transplantation. A ddcfDNA threshold value of 0.88% (mean + 2SD) was determined in kidney transplant recipients. Recipients that did not reach this threshold ddcfDNA value within 10 days after transplantation showed a higher ddcfDNA% on the first day after transplantation and demonstrated a higher individual baseline ddcfDNA%. Conclusion In conclusion, plasma ddcfDNA fractions decreased exponentially within 10 days after transplantation to a ddcfDNA threshold value of 0.88% or less. To investigate the role of ddcfDNA for rejection monitoring of the graft, future research is needed to determine causes of ddcfDNA% increases above this threshold value.
Language
English
Source (journal)
PLoS ONE
PLoS ONE
Publication
2018
ISSN
1932-6203
Volume/pages
13 :12 (2018) , 16 p.
Article Reference
e0208207
ISI
000452307600033
Pubmed ID
30521549
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Non-invasive methods for the early detection of renal allograft rejection: the role of donor-derived (circulating) cell free DNA.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 10.12.2018
Last edited 15.10.2021
To cite this reference