Uniform nanosecond pulsed dielectric barrier discharge plasma enhances anti-tumor effects by induction of immunogenic cell death in tumors and stimulation of macrophages

Lin, Abraham; Truong, Billy; Pappas, Arthur; Kirifides, Lawrence; Oubarri, Ahmed; Chen, Shuyang; Lin, Shaojun; Dobrynin, Danil; Fridman, Gregory; Fridman, Alexander; Sang, Nianli; Miller, Vandana

doi:10.1002/PPAP.201500139

Title

Uniform nanosecond pulsed dielectric barrier discharge plasma enhances anti-tumor effects by induction of immunogenic cell death in tumors and stimulation of macrophages

Author

Lin, Abraham

Truong, Billy

Pappas, Arthur

Kirifides, Lawrence

Oubarri, Ahmed

Chen, Shuyang

Lin, Shaojun

Dobrynin, Danil

Fridman, Gregory

Fridman, Alexander

Sang, Nianli

Miller, Vandana

Abstract

Several have shown plasma to be efficacious against cancer cells in vitro and in vivo with minimal damage to non‐cancerous cells and tissue. Most are focused on direct influence of plasma on tumor cells. However, the body's immune system also plays a crucial role in the control of cancer. A new concept of immunogenic cell death (ICD) has been emerging, where through this modality of cell death, an immune response may be stimulated. The goal of the presented study was to explore a regime of plasma treatment that induces ICD in tumor cells and stimulates anti‐tumor effects in macrophages‐ a key immune cell type involved in the initiation of immunological responses. Here, we show that treatment with uniform nanosecond pulsed dielectric barrier discharge (nspDBD) plasma directly activated anti‐tumor effects in macrophages. We further show macrophage activation following the expression of plasma‐induced damage‐associated molecular patterns (DAMPs) in tumor cells, characteristic of ICD.

Language

English

Source (journal)

Plasma processes and polymers. - Weinheim

Source (book)

2nd International Workshop on Plasma for Cancer Treatment (IWPCT), 2015, Nagoya, Japan

Publication

Weinheim : 2015

ISSN

1612-8850

DOI

10.1002/PPAP.201500139